Journal: STAR Protocols

Article Title: Protocol to establish turkey oviductal organoids as an in vitro model

doi: 10.1016/j.xpro.2024.103384

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Article Snippet: 48-well non-treated tissue culture plates , Celltreat Scientific Products , Cat# 229548.

Techniques: Recombinant, Saline, Membrane, Plasmid Preparation

Journal: Biomaterials

Article Title: Surfaces modified with nanometer-thick silver-impregnated polymeric films that kill bacteria but support growth of mammalian cells

doi: 10.1016/j.biomaterials.2009.09.092

Figure Lengend Snippet: Viability of NIH 3T3 cells on PEM-coated glass surfaces impregnated with silver nanoparticles is silver-dose dependent. NIH 3T3 cells (1.5 × 104 per well) were incubated on PEM-coated glass coverslips placed in 96-well plates for 24 h and then labeled with live/dead fluorescent stains. PEMs were prepared using solutions of PAA with pH values ranging from 2.5 to 7.5 to vary the loading of silver. Data presented as mean ± S.D. with n = 6.

Article Snippet: The plasma oxidized glass coverslips (5 mm diameter) were placed at the bottom of the wells of a 96-well plate (BD Falcon ® Polystyrene, non-tissue culture treated 96-well plates, BD, Franklin Lakes, NJ), and PEMs were assembled by sequentially placing solutions of either PAH or PAA in the wells for 10 min.

Techniques: Incubation, Labeling

Journal: Biomaterials

Article Title: Surfaces modified with nanometer-thick silver-impregnated polymeric films that kill bacteria but support growth of mammalian cells

doi: 10.1016/j.biomaterials.2009.09.092

Figure Lengend Snippet: PEMs impregnated with as little as 0.39 ± 0.03 μg/cm2 silver are bactericidal for S. epidermidis. PEM-coated glass coverslips were placed in 96-well plates and incubated with 107 CFU of S. epidermidis in 100 μl PBS buffer for 8 h with shaking (100 rpm) at 37 °C After incubation, bacteria were rinsed off the test wells and plated on agar to estimate viable cell counts (CFU/well). (a) PEMs assembled using PAA at pH 2.5 and impregnated with 23.6 ± 1.1 μg/cm2 silver using a 5 mm silver nitrate solution. (b) PEMs assembled using PAA at pH 7.5 and impregnated with 0.39 ± 0.03 μg/cm2 silver using a 5 mm silver nitrate solution. In addition, results are shown for PEMs were impregnated with concentrations of silver nitrate solutions that were less than 5.0 mm, as indicated on x-axis of the graph. The loadings of silver nanoparticles in the PEMs prepared using the low concentrations of silver were below the detection limit of ICP-ES. Overall, the data demonstrates silver-dose dependent bactericidal activity. Data presented as mean ± standard deviation (n = 3).

Article Snippet: The plasma oxidized glass coverslips (5 mm diameter) were placed at the bottom of the wells of a 96-well plate (BD Falcon ® Polystyrene, non-tissue culture treated 96-well plates, BD, Franklin Lakes, NJ), and PEMs were assembled by sequentially placing solutions of either PAH or PAA in the wells for 10 min.

Techniques: Incubation, Activity Assay, Standard Deviation

Journal: STAR Protocols

Article Title: Preparation of chaperone-loaded neural stem cell-derived extracellular vesicles to reduce protein aggregation in Huntington’s disease cellular models

doi: 10.1016/j.xpro.2023.102134

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Article Snippet: CostarTM 6-well Clear TC-treated Multiple Well Plates, Individually Wrapped, Sterile , Corning , 3516.

Techniques: Recombinant, Saline, DC Protein Assay, Polymerase Chain Reaction, Plasmid Preparation, Software, Fluorescence, Microscopy, Imaging, Western Blot, Membrane, Blocking Assay, Sterility